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1. HPLC Troubleshooting

Problem System Related Low unsteady system pressure High system pressure Noisy fluctuating drifting baseline Regular pulsing of the baseline The Chromatogram Tailing peaks Fronting tailing peaks Reason Leak Air in pump head Dirt in check valve check whether valve cannot close Blockage contamination Blockage precipitated buffer salts can happen when the system or user suddenly changes mobile phase composition from high organic
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