IRAM S CY SO EREN fy M Discovery is yours NA CELLECTA Pooled Lentiviral shRNA Library Screening Reference Manual V1 10 22 2014 www cellecta com Cellecta 2014 All Rights Reserved Cellecta Pooled Library Screening www cellecta com Reference Manual Contents A Introduction to Pooled shRNA Libraries cceecee cece eee eee ee eee mmm menn 4 B Design and Quality Control of Cellecta s shRNA Libraries ccececeeeee e
shRNA expression Cloning Kit User Manual for making lentiviral and non lentiviral shRNA expression clones Cat Product Name Amount Application LTSH GB _ pEco Lenti H1 shRNA 1 kit Make shRNA expression GFP Bsd cloning kit lentivector with selection marker LTSH GP _ pEco Lenti H1 shRNA 1 kit Make shRNA expression GFP Puro cloning kit lentivector with selection marker LTSH RB _ pEco Lenti H1 shRNA 1 kit Make shRNA expression RFP Bsd cloning kit lentivect
technologies SStransomic PLATINUM Select MLP Retroviral shRNA mir TRH1000 TRM1001 TRHS1000 TRMS1001 TRHK1000 TRMK1001 TRH5000 TRM5000 Overview PLATINUM Select MLP Retroviral microRNA adapted short hairpin RNA shRNA mir are next generation vector based RNAi triggers generated using the proprietary shERWOOD Algorithm developed in Dr Greg Hannon s laboratory at Cold Spring Harbor Laboratories Based on the functional testing of 270 000 shRNA sequences using a high t
SBI System Biosciences pGreenPuro shRNA Cloning and Expression Lentivector Cat s SI505A 1 User Manual Store kit at 20 C on receipt A limited use label license covers this product By use of this product you accept the terms and conditions outlined in the Licensing and Warranty Statement contained in this user manual ver 081124 pGreenPuro shRNA Cloning and Expression Lentivector Cat 81505A 1 Contents l Introduction and Background
A lt ie A LU 1 red e du DM gp ASF Jj a 2 us LS AET ESY T 4 1 qf Discovery is yours 4 CELLECTA Cloning of shRNA and sgRNA Templates into Cellecta Expression Vectors User Manual V5 7 16 15 ATA Ae UCSTed Wee 11 shRNA amp sgRNA Cloning www cellecta com Contents As BACKECOUIN e PH 2 B Required Materials rre ore Ue ei ne Ue PEE Qe ER ES a EUNT NEATE Yee geben e PUn o 2 C Design o
Cellecta Inc 320 Logue Ave Mountain View CA 94043 CELLECTA Ht covets E Cloning of shRNA Templates into shRNA Expression Vector User Manual Table of Contents v 4 Background Required Materials Cloning of shRNA Template Oligonucleotides into shRNA Expression Vector Identify clones with the target shRNA template Purify Plasmid shRNA Construct Technical Support Safety Guidelines References rammMooW DY NOM KR RWYN HB e A Background Th
SBI System Biosciences pSIH H1 shRNA Cloning and Expression Lentivectors Cat s SI500A 1 SI501A 1 SI502A 1 User Manual Store kit at 20 C on receipt A limited use label license covers this product By use of this product you accept the terms and conditions outlined in the Licensing and Warranty Statement contained in this user manual ver 4 080514 pSIH H1 shRNA Cloning and Expression Lentivectors Contents Vi 888 266 5066 Toll Free Int
Lentiviral shRNA expression Cloning Kit User Manual for making shRNA expression lentivectors Cat Product Name Amount Application LTSH GB pEco Lenti H1 kit Make shRNA expression shRNA GFP Bsd lentivector with cloning kit selection marker LTSH GP pEco Lenti H1 l kit Make shRNA expression shRNA GF P Puro lentivector with cloning kit selection marker LTSH RB pEco Lenti H1 1 kit Make shRNA expression shRNA REP Bsd lentivector with cloning kit selection m