invitrogen pcDNA4 TO E Echo Adapted Expression Vector For cloning a gene of interest using the Echo Cloning System and inducible expression in mammalian cells using the T REx System Catalog nos ET460 XX Version F 28 October 2010 25 0327 11 Table of Contents Table of Contents daa eames iii Kit Contents and Storage iiem ea e anaE E his EEE EEEE A EEEE Eaa E EEEE v INtrOdUCHON ii 1 OVENI Wai id AA A A a a A A AAA EA di 1 MeIh ds n usnuisunsnea inn
invitrogen NativePure pcDNA Gateway Vector Kit For expression and purification of N and C terminal biotinylated fusion proteins and associated complexes in mammalian cells Catalog nos BN3002 BN3006 Version B 28 October 2010 25 0892 ii Table of Contents Experienced Users Procedure cscccccsssscscscccesssetetesesesssnsnsesescsescsceeeeeesesesesesnsnenssesesesesnaanesessseessesesesnananes iv K it Cofitentssand Stora ges asics sans aan an seh EAA E v
invitrogen pcDNA 3 1 pcDNATM3 1 Catalog nos V790 20 and V795 20 Version K 10 November 2010 28 0104 11 Table of Contents Important OIM ON AA AAA AAA v ECOS ELO ES AA ea via E E A ea vi Methods cr aso 1 DO NN eG Roe 1 Cloning into PeDNA MI Eee 2 Transtech A O A es 6 CrestingstabletellLmes ae 7 Appendix aaa 10 BEDNAPS T Meco iMpenVecnienne 10 PCDI MAA ad 12 Technical Supporto cassia acute a eee a E 13 Purchaser Noticas aa i a ia Eina aiiin 13
invitrogen pcDNA 4 myc His A B and C Catalog no V863 20 Rev Date 27 October 2010 Manual part no 25 0236 MAN0000078 ii Table of Contents Kit Contents nd Storage sun nennen deed send iv Introduction ae 1 Product ONE Wi isa 1 MethodsS A eneen edad 2 Cloning into ppDNA 4 myc His A B and Ei silanen ans 2 Transtection and AN alysis odian lt ii pi 6 Creating Stable Cell Lines an danseres taaan ava e eaea caera dolencia das benee beard 8 APpendix N
invitrogen pcDNA 5 FRT TO Inducible expression vector designed for use with the Flp In T REx System Cat no V6520 20 Version G 11 November 2010 25 0368 Table of Contents Kit Contents and Storage assssevarsannsansnonen on saas nrncnancnno diran cnica idad v Methods En 1 OVERVIEW rt ccs a e a aa a IAEA a a a CIA AI cot tans aE 1 Cloning into pPDPNA 5 FRT TO touren eiiiai an er SLR ERBE sah lei 4 Transfeetion nurse en a a ls il 7 APpENdIX NN aaa 9 PEDNAFS ER
invitrogen pcDNA 6 myc His A B and C Catalog no V221 20 Rev date 9 November 2010 Manual part no 25 0233 MAN0000076 ii Table of Contents Kit Contents and Gtorage ue iv ee ETC v Introduction ee 1 KO Dee Fedre nr EE EE Ee 1 Methoden ee ee are nn 2 Cloning into ppDNA 6 myc His A B and ii A eneen 2 Transfection and Analysis ege deed nis alu 7 Creating stable Cell EE EE 10 elt TEE 14 BEDNA 6 1 TIS Ves 14 FONN GS TAG 16 BEE E E 17 P rchaser N ti
invitrogen pcDNA 4 TO myc His A B and C Expression vectors with C terminal tags designed for use with the T REx System Catalog no V1030 20 Revision date 13 November 2010 Manual part no 25 0287 MAN0000117 11 Table of Contents Contents and Store ici a LENE iv Accessory Products ze testei berri e O tinae aria Gat eve oi ida v Introduction cc ds 1 System Overview aat mrien IR neee de el dane AAA ecb 1 MethodS a 4 Cloning into peDNATA TO mye His A BA
invitrogen pcDNA 3 2 V5 DEST and pcDNA 6 2 V5 DEST Gateway Vectors Gateway adapted destination vectors for cloning and expression of C terminal V5 fusion proteins in mammalian cells Catalog nos 12489 019 and 12489 027 Version E 27 October 2010 25 0500 Table of Contents Important Information mserevserrrrorerersererrererersersrreverseversrsevessaversssevessevesseversssenersns v Accessory Prod ucts usunn uuavsnneginanansinnemengnesedees vi LEIALO Lo
Invitrogen pcDNA 3 1 myc His A B and C Catalog no V800 20 Rev Date 28 October 2010 Manual part no 28 0137 MAN0000641 ii Table of Contents Kit Contents dnd Storage teerde edet e pdt deca ahh iv INTO GU CHOI sicario H 1 OLIVO EE 1 kele CEE 2 Cloning into pcDNA 3 17 myc His A B and C teen a la 2 Transformation and Transfection nanne ensen ennvenseenernvenneenvenvennvenvensennvenserneenvenneenvencenneenvennennenn 5 ABPPENdIX dum
Invitrogen pcDNA 3 1 Zeo pcDNA 3 1 Zeo For high level stable and transient expression in mammalian hosts Catalog nos V860 20 and V865 20 Version J 12 November 2010 28 0110 Table of Contents Kit Contents and storage dn Gasa een v Accessory Products ise RE eH REI GRE de vi Introduction ss 1 OVER DEB NE PUER M dan dan danken eee ped tah ue 1 Methods fte DRE 2 Cloning into pcDNA 3 1 Zeo un an