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1. PCR clean-up Gel extraction

PCR clean up Gel extraction User manual NucleoSpin Extract II September 2005 Rev 02 MACHEREY NAGEL MN Protocol at a glance Rev 02 NucleoSpin Extract II Gel extraction PCR clean up 1 Excise DNA fragment Z 2 Gel lysis 200 pI NT Adjust binding conditions 100 mg 200 pI NT 100 pl 50 C 5 10 min 3 Bind DNA 1 min 11 000 x g 4 Wash silica membrane 600 uI NT3 1 min 11 000 x g 5 Dry silica membrane 2 min 11 0
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