CLONTEGH Innovative Tools to Accelerate Discovery Advantage 2 PCR Enzyme System User Manual PT3281 1 PR11009 Published 23 January 2001 Storage conditions 20 C FOR RESEARCH USE ONLY Advantage 2 PCR Enzyme System User Manual Table of Contents l Introduction 3 ll List of Components 6 Ill Additional Materials Required 7 IV Advantage 2 PCR Protocol 8 A General Considerations 8 B Control PCR Reactions 11 C Recommended Cycling Parameters 12 D
CLONTECH Innovative Tools to Accelerate Discovery MATCHMAKER GAL4 Two Hybrid System 3 amp Libraries User Manual PT3247 1 PR94575 Published 22 June 1999 See List of Components for storage conditions FOR RESEARCH USE ONLY MATCHMAKER GAL4 Two Hybrid System 3 amp Libraries User Manual Table of Contents Introduction ll Overview A Yeast Two Hybrid Screen V Vi VII VIII xI XII XIII Lists of Components V Additional Material
CLONTECH Innovative Tools to Accelerate Discovery TALON Metal Affinity Resins User Manual PT1320 1 PR16704 Published 01 October 2001 See List of Components for storage conditions FOR RESEARCH USE ONLY TALON Metal Affinity Resins User Manual Table of Contents Vil VIII IX X xI xII Introduction List of Components Additional Materials Required Buffers for TALON Purification amp Buffer Kits Transformation amp Protein Expre
User Manual Yeast Protocols Handbook Clontech FOR RESEARCH USE ONLY United States Canada 800 662 2566 Asia Pacific 1 650 919 7300 PT3024 1 PR973283 Europe Published July 2009 33 0 1 3904 6880 Japan 481 0 77543 6116 Clontech Laboratories nc ATakara Bio Company 1290Terra Bella Ave Mountain Vievv CA 94043 Technical Support US E mail tech O clontech com www clontech com Yeast Protocols Handbook Table of Contents VI VII
YEASTMAKER Yeast Plasmid Isolation Kit Protocol at a Glance PT3049 2 Please read the User Manual before using this Protocol at a Glance This abbreviated protocol is provided for your convenience but is not intended for first time users Note that the corresponding steps may be numbered differently here than in the User Manual Resuspended yeast cells 50 ul 1 Add 10 ul lyticase 2 Incubate at 37 C for 30 60 min 3 Vortex Prespin CHROMA SPIN 1000 4 Add 10 p
CLONTECH Innovative Tools to Accelerate Discovery AdvanTAge PCR Cloning Kit User Manual PT3067 1 PR01082 Published 24 March 2000 Catalog K1901 1 See List of Components for storage conditions FOR RESEARCH USE ONLY AdvanTAge PCR Cloning Kit User Manual Table of Contents I Introduction 3 ll List of Components 6 Additional Materials Required 8 IV PCR Amplification 10 V Cloning into pT Adv 12 VI Transformation 14 Vil Troubleshooting Guide
CLONTECH Innovative Tools to Accelerate Discovery BacPAK Baculovirus Expression System User Manual PT1260 1 PR95847 Published 14 May 1999 Catalog K1601 1 See List of Components for storage conditions FOR RESEARCH USE ONLY o Nu na 10 10 11 11 13 14 15 15 17 17 18 18 18 19 19 21 22 24 24 24 25 25 26 27 27 BacPAK Baculovirus Expression System User Manual Table of Contents Introduction List of Components Additio
CLONTECH Innovative Tools to Accelerate Discovery HAT Protein Expression and Purification System User Manual PT3250 1 PR16705 Published 01 October 2001 Catalog K6050 1 See List of Components for storage conditions FOR RESEARCH USE ONLY I _ Introduction 4 ll List of Components 8 lll Additional Materials Required 9 IV Cloning Recombinant Proteins Containing HAT 10 A Use of HAT Purification Sequence in Other Vectors 10 B Transformation of Host Cells
CLONTECH Innovative Tools to Accelerate Discovery Antibody Microarrays User Manual PT3648 1 PR23092 Published 20 March 2002 See List of Components for storage conditions FOR RESEARCH USE ONLY Antibody Microarrays User Manual Table of Contents I Introduction amp Protocol Overview ll List of Components lll Additional Materials and Equipment Required IV General Considerations V Antibody Array Detection Protocol A B C D E F Extract