Metagenomics Workshop Lead by Regina Lamendella Juniata College lamendella juniata edu 814 641 3553 Acknowledgements I would like to thank Ms Erin McClure for her help in developing many of these tutorials and for her help in preparing this document Table of Contents 16S RRNA GENE AND ITS LIBRARY PREPARATION A Background B Library Preparation 1 16S rRNA gene Illumina tag itag PCR 2 ITS Illumina tag itag PCR C Check PCR Amplification 1 Pool replicate sam
Metagenomic Sample Analysis Standard Operating Procedures for fossil fuel environments HYDROCARBON METAGENOMICS Contents 1 2 3 5 6 A EEUU ERU PT 4 1 1 Metadata SNC 4 1 2 Sample Prdctionatiorisse eo treo uie az 4 Ns A AO 5 2 1 DNA Sample FractionatiOi asiatico 7 COMMUNITY Ana SIS an 8 3 1 PGR AMPINICICON a A date rcu cds bacs E Liu freed 8 Procedure PCR amplification and barcoding cccoocccnconocnnnonaconnnnnncnnnnnncnnnonaronnonanonnnnnnonnnon