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1. RNA isolation

RNA isolation User manual NucleoSpin RNA NucleoSpin RNA Midi February 2013 Rev 15 MACHEREY NAGEL www mn net com MN RNA isolation Protocol at a glance Rev 15 wm ma NucleoSpin RNA NucleoSpin RNA Midi 1 Homogenize sample e 30 mg c 100 mg 2 Lysecells Filtrate lysate Adjust RNA binding conditions 5 Bind RNA 6 Desalt silica membrane Digest DNA Wash and dry silica membrane 9 Elute highly pure RNA 350 uL R
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